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. 2017 May 23;8:15349. doi: 10.1038/ncomms15349

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Copyright © 2017, The Author(s)

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(a) LC–MS analysis of the in vitro reaction of C_2(BbBEAS) and C_3(BbBEAS) with S1 and S2. The left panel is the negative control with inactivated C₂ and C₃, while the right is the actual reaction. The HPLC traces are shown on the top, followed by the EICs of the intermediates and final product. (i)–(v) are EICs at m/z 642 (i), 784 (ii), 262 (iii), 641 (iv) and 903 (v), respectively. (b) Enlarged view of the region of 30.1–34.4 min of the HPLC traces of the negative control (i), reaction (ii) and the standard of 1 (iii).