Figure 4.

Changes in adipocytokine secretion from adipose tissue upon dietary switch. (A) FFA concentrations in CM of adipose tissue (100 mg tissue/mL medium). (B) Total FFA production by epididymal fat pads. (C) Adipocytokine array was used to screen for changes induced by dietary switch in the secretion of adipocytokines by cultured adipose tissue fragments. The levels of each adipocytokine were quantified by densitometry and expressed as fold of HFF for each adipokine. The detected factors were divided to those which qualitatively changed similarly (black bars) or differently (white bars) to their change induced in HFF adipose tissue by incubation with p38MAPK + JNK inhibitors (results of this analysis is presented in Supplementary Fig. 1). Results are mean ± s.d. of 2–3 independent experiments.