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. 2017 May 23;8:15534. doi: 10.1038/ncomms15534

Figure 1. USP13 interacts with STING.

Figure 1

(a) Immunoprecipitation (IP, with anti-FLAG) and immunoblot analysis (with anti-FLAG and anti-HA) of HEK293 cells transfected with plasmids encoding FLAG-tagged USPs and HA-STING for 24 h. (b) Luciferase reporter assays analysing IFN-β promoter activity (top) and immunoblot assay of FLAG-tagged USPs, Myc-STING and HA-cGAS of HEK293 cells transfected with the indicated plasmids for 24 h. (c,d) Immunoprecipitation (with anti-HA) and immunoblot analysis (with anti-FLAG or anti-HA) of HEK293 cells transfected with plasmids encoding HA-STING and FLAG-tagged USP13 or USP13 truncates for 24 h (c) or transfected with plasmids encoding FLAG-USP13 and HA-STING or STING truncates for 24 h (d). (e) Immunoprecipitation (with anti-STING or IgG as a control) and immunoblot analysis (with anti-STING or anti-USP13) of BMDCs (left) and MEFs (right). Data are representative of three independent experiments (mean±s.d. in b). See Supplementary Fig. 11 for uncropped immunoblots.