Table 1. Cryo-EM data collection, reconstruction and refinement statistics.
O PanAsia | O1M | |
---|---|---|
Data collection and reconstruction | ||
Voltage (kV) | 300 | 300 |
Movies | 360 | 285 |
Frames | 25 | 25 |
Dose rate (e− per pixel per s) | 8 | 8 |
Pixel size (Å) | 1.35 | 1.35 |
Total dose (e− Å−2) | 18 | 18 |
Defocus (μm) | 1.5–3.0 | 1.5–3.0 |
Particles* | 13,483 | 1,649 |
Resolution (Å)† | 3.1 | 3.5 |
B-factor (Å2)‡ | −120 | −92.9 |
Model refinement | ||
Fo-Fc correlation | 0.84 | 0.82 |
Protein atoms | 5,149 | 5,149 |
R.m.s.d., bonds (Å) | 0.01 | 0.01 |
R.m.s.d., angles (°) | 0.95 | 0.77 |
Clash score, all atoms (percentile) | 9.45 | 11.53 |
Rotamer outliers (%) | 0.0 | 0.0 |
Ramachandran outliers (%) | 0.0 | 0.0 |
R.m.s.d., root mean squared deviation.
*The number of particles used in the final reconstruction is given.
†Resolution as estimated by Fourier shell correlation with 0.143 threshold.
‡B-factor used for map sharpening.