Table 1. Cryo-EM data collection, reconstruction and refinement statistics.
| O PanAsia | O1M | |
|---|---|---|
| Data collection and reconstruction | ||
| Voltage (kV) | 300 | 300 |
| Movies | 360 | 285 |
| Frames | 25 | 25 |
| Dose rate (e− per pixel per s) | 8 | 8 |
| Pixel size (Å) | 1.35 | 1.35 |
| Total dose (e− Å−2) | 18 | 18 |
| Defocus (μm) | 1.5–3.0 | 1.5–3.0 |
| Particles* | 13,483 | 1,649 |
| Resolution (Å)† | 3.1 | 3.5 |
| B-factor (Å2)‡ | −120 | −92.9 |
| Model refinement | ||
| Fo-Fc correlation | 0.84 | 0.82 |
| Protein atoms | 5,149 | 5,149 |
| R.m.s.d., bonds (Å) | 0.01 | 0.01 |
| R.m.s.d., angles (°) | 0.95 | 0.77 |
| Clash score, all atoms (percentile) | 9.45 | 11.53 |
| Rotamer outliers (%) | 0.0 | 0.0 |
| Ramachandran outliers (%) | 0.0 | 0.0 |
R.m.s.d., root mean squared deviation.
*The number of particles used in the final reconstruction is given.
†Resolution as estimated by Fourier shell correlation with 0.143 threshold.
‡B-factor used for map sharpening.