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. 2017 May 23;8:15411. doi: 10.1038/ncomms15411

Figure 1. EDE mAbs can stabilize DENV envelope dimers.

Figure 1

(a) Binding of anti-FLE and anti-EDE mAbs to monomeric sE protein was determined by direct ELISA with sE coated to the ELISA plate. Results are expressed as mean of binding in arbitrary units (AU) from three independent experiments. The mAbs used in b are indicated by dots of the same colour. (b) The ability of selected mAbs to bind and assemble dimers was assessed by indirect ELISA. ELISA plates were coated with four anti-EDE mAbs and one anti-FLE mAb control, which binds monomeric sE. Plates were then incubated with a titration of soluble Strep-tagged sE monomer, bound sE was revealed using ALP-labelled StrepTactin. The data are shown as mean±s.e.m. from three independent experiments. (c) SEC/MALS analysis of isolated DENV2 sE, isolated Fab fragments and DENV2 sE with anti-EDE1 Fab C8 (left panel) and anti-EDE2 Fab A11 (right panel) mAbs. The molecular weight determined by MALS is indicated, corresponding to the y axis on the left. The ultraviolet absorbance was normalized such that the highest peak is set to 1 (y axis on the right).