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. 2017 May 23;8:15504. doi: 10.1038/ncomms15504

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The hydrophobic nature of surfactant peptides rSP-C33Leu and rKL4 allows for purification through salt precipitation and ethanol extraction. The figure describes each step in the purification protocol and further explains the denotations P1, S1, P2, S2 and T in Fig. 4e,f.