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. 2017 Jun 2;10:143. doi: 10.1186/s13068-017-0833-x

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — TLC analysis of mannans and manno-oligosaccharides hydrolysed by mRmMan5A. mRmMan5A (5 U mL⁻¹) was incubated with 1% (w/v) mannobiose, mannotriose (a), mannotetrose (b), LBG (c), or konjac powder (d) in 50 mmol L⁻¹ McIlvaine buffer (pH 4.5) at 50 °C for 12 h. Incubation times (hours or minutes) are indicated. Lane Mn standard of manno-oligosaccharides