Table 2.

Kinetic parameters of mRmMan5A and RmMan5A (mean ± SD)

Procedure	V max (μmol min‒1 mg−1)	K m (mg mL‒1)	k cat (s‒1)	k cat/K m (mg s‒1 mL‒1)
LBG
mRmMan5A	10,154.9 ± 293	3.27 ± 0.22	7446.9	2277.3
RmMan5A	3192.5 ± 75	3.37 ± 0.18	2341.2	694.7
Guar gum
mRmMan5A	1516.3 ± 47	7.06 ± 0.42	1112.0	157.5
RmMan5A	402.9 ± 11	7.12 ± 0.39	294.8	41.4

Enzymatic reactions were carried out in 50 mmol L⁻¹ McIlvaine buffer (pH 4.5) at 65 °C for 5 min using LBG or guar gum as substrate