Fig. 3.

ATF3 facilitates embryo attachment in vitro. a Ishikawa cells were transduced with Ad-LacZ and Ad-ATF3-His at 0 or 40 MOI for 48 h. b Ishikawa cells were exposed to E₂ and MPA for 24 h after 48 h transfection with 0 or 100 nM si-CTL or si-ATF3. Adhesion experiments were performed to assess BeWo spheroid attachment to the Ishikawa cell monolayer. The data represent the results of 3 independent experiments. ANOVA was used to compare the percentage of the attached spheroids in each treatment with that of the control. **p < 0.01 vs. the Ad-LacZ group. *p < 0.05 vs. the si-CTL group