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. 2017 Jun 2;13:154. doi: 10.1186/s12917-017-1074-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a Skin of a flipper. Parasite situated over the epidermis showing abundant granulated brownish material into its gut (arrow). Hematoxylin and eosin; scale bar = 200 μm. b Skin of a flipper. A parasite attached to the keratinized layer of the epidermis. The transversal section of the parasite allows observing brown laminated material that resembles keratin and small black spots that resemble melanin granules in gut content (arrow). Hematoxylin and eosin; scale bar = 100 μm. c Longitudinal and d cross sections of a parasite. Gut content reacts positively for cytokeratin. Anti-cytokeratin monoclonal antibody and ABC; scale bars = 200 and 100 μm, respectively