Table 3. IR, MIR and viral titers of oviposited females, eggs and newly emerged females in the second gonotrophic cycle of infectious Ae. aegypti.

Exp.	Strain	Number tested	Number positive	IRa (%)	MIRb (%)	log10 RNA copies per mL
Oviposited femalesc	HK	14	14	100	—	9.48±1.12
	RL	10	10	100	—	8.05±2.77
Eggs (30 per pool)	HK	24	13	54.17	1.81	4.41±0.82
	RL	23	12	52.17	1.74	5.19±0.71
Salivary gland of newly emerged femalesd	HK	47	1	2.13	—	4.77±0.00
	RL	43	1	2.33	—	3.45±0.00

Abbreviation: Zika virus, ZIKV.

^aIR (infection rate) is calculated as the ratio of the number of ZIKV-positive individuals or pools to the total number of individuals or pools tested.

^bMIR (minimum infection rate) is the ratio of the number of positive pools to the total number of individuals (eggs) tested.

^cOviposited females were mosquitoes that laid eggs in the second gonotrophic cycle. Ten to fifteen individuals were randomly removed from cages and analyzed.

^dEggs obtained from the second gonotrophic cycle were reared under standard laboratory conditions through the larvae and pupae stages until a new generation of adults emerged. The salivary glands of newly emerged females were dissected and analyzed.