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. 2017 Apr 12;6(4):e17. doi: 10.1038/emi.2017.3

Table 2. Effect of mutations A222V and G228S of influenza A(H6N6) virus hemagglutinin on binding affinity to erythrocytes from various animalsa.

Virus Mean hemagglutination titerb
  Turkey Chicken Guinea pig Horse
Types of erythrocytes        
 rgH6N6 × PR8-222V/228S 32 32 32 32
 rgH6N6 × PR8-222A/228S 32 32 16 32
 rgH6N6 × PR8-222V/228G 32 64 <2 2
 rgH6N6 × PR8-222A/228G 32 64 <2 <2
 rgH6N6 × pdm09-222V/228S 32 32 32 16
 rgH6N6 × pdm09-222A/228S 32 32 32 32
 rgH6N6 × pdm09-222V/228G 32 64 <2 <2
 rgH6N6 × pdm09-222A/228G 32 128 <2 2
a

rg indicates reverse genetics.

b

All virus concentrations were normalized to a hemagglutination titer of 32 in turkey erythrocytes. Each assay was repeated three times, and in each case, the standard deviation was 0.