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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Mol Cancer Res. 2017 Feb 24;15(6):670–682. doi: 10.1158/1541-7786.MCR-16-0411

Fig. 1. TNBC cell morphology changes upon NEDD9 and ROCK inhibition.

Fig. 1

(A) Western blot analysis of NEDD9 expression in MDA-MB-231, HCC1143, and Hs578T treated with multiple siRNAs. (B) Brightfield images of MDA-MB-231, HCC1143, and Hs578T cells treated with siCon or siNEDD9 in 3D collagen I and (C) cell elongation quantified as cell length/width (40 cells/group). (D) Brightfield images of BT549, HCC1395, and SUM159 cells expressing siCon, siNEDD9, or CA-RhoA, (E) cell elongation quantified as cell length/width (100 cells/group), and (F) western blots of NEDD9 knockdown and CA-RhoA expression. (G) Brightfield images of TNBC cells, vehicle or Y-27632 treatment and (H) cell elongation quantified as cell length/width (40 cells/group). ns, not significant; *p<0.05, student’s t-test vs control.