Table 1.
List of selected genes, PCR primers, and predicted size
| Fosmid name | Predicted enzyme (Gene encoding) | Gene no. | Primers | Restriction enzymes | Size (bp) | Approx. size (kDa) | Closet hit (identity/coverage) |
|---|---|---|---|---|---|---|---|
| NT2-2 | β-Galactosidase | 1 | FP-TACgaattcACGGCATCAGCGCAATAGTC RP-AGggatccCGCTTCGCAACTGAGGTTAC |
EcoRI BamHI |
3146 | 116 | Enterobacter hormaechei (74/81%) |
| T5-5 | β-Xylosidase | 2 | FP-GCGaagcttGTGTAGTCTTTGCCCATCTC RP-CAGggatccCGCTTCGCAACTGAGGTTAC |
HindIII BamHI |
2410 | 85 | Enterobacter mori (83/90%) |
| T4-1 | β-Xylosidase | 3 | FP-GCGgaattcCACCAGATTCAGCCCTACAG RP-CAGaagcttTGTAGTCTTTGCCCATCTCC |
EcoRI HindIII |
2439 | 86 | Enterobacter mori (83/90%) |
| 10BT | Transcription regulator | 4 | FP-GCGggatccGCACAACAAGATGGCTTTAC RP-CAGctcgagTAGTCGCATTTTGACGGAAC |
BamHI XhoI |
627 | 22 | Klebsiella oxytoca (76/87%) |
| Inner membrane protein | 5 | FP-GCGaagcttGTGCAAAGTATCGCTTTAAC RP-CAGggatccAGCAGCATGAATAAGGACAC |
HindIII BamHI |
1262 | 45 | Enterobacter cloacae (79/88%) | |
| NT18-17 | Aquaporin | 6 | FP-GCGaagcttGGGATCGGCTAGATCTTGAG RP-CAGggatccCAAGCGTCATGAATAAACTG |
HindIII BamHI |
673 | 22 | Hyphomonas neptunium (65/80%) |
| Beta-hexosaminidase | 7 | FP-GCGggatccTTTGCGGAGCCTACTCCTTC RP-CAGaagcttTTACAATGGCATCTGATTGG |
BamHI HindIII |
1944 | 70 |
Rhizobium leguminosarum
3841 (50/64%) |
|
| Hypothetical protein | 8 | FP-GCGggatccCCGGATACTCCACACAAAGG RP-CAGctcgagTTATCGCAAGTCGATCAAGG |
BamHI XhoI |
1134 | 37 | Hyphomicrobium denitrificans (56/71%) |