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. 2017 Jun 2;9:60. doi: 10.1186/s13148-017-0360-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — ZAR1 expression pattern, missing expression in lung cancer and promoter hypermethylation in lung cancer cell lines. ZAR1 expression in lung cancer cell lines A427, A549, HTB171, H322, H358 and HeLa (cervix carcinoma) versus normal tissue panel (breast, kidney, liver, lung and heart) by RT-PCR and semi-/quantitative PCR were determined. a, b Expression levels were normalised to ß-ACTIN (ACTB) and to normal lung tissue (=100%). Promoter hypermethylation of lung cancer cell lines, HEK and HeLa cells by COBRA analysis c and pyrosequencing for quantification d are shown. PCR and digestion products were separated on 2% TBE agarose gel with 100 bp marker M (m methylated, pm partially methylated, pos. positive control, neg. negative control; + TaqI digested, − mock digested)