Table 3.

Fermentation performance parameters in all the pH-controlled experiments carried out in this work

Engineered strain	Fermentation medium	pH	Temperature (°C)	Maximum ethanol concentration (g/l)	Fermentation time (h)	Fermentation yield (% of theoretical maximum yield)	Initial lactose (g/l)	Volumetric productivity (g/l/h)
W-pL13	LB	7.0	30 °C	28.9	25	70	76.5	1.1
W-pL13	WP	7.0	30 °C	13.7	94	60	42.3	0.15
W-pL13	WP	6.6	30 °C	17.6	73	65	50.1	0.23
W-pL13	WP	7.0	37 °C	13.0	46.5	54	44.3	0.27
W-pL13	WPa	7.0	37 °C	12.8	50	45	52.4	0.26
W-pL13	CWP	6.6	37 °C	35.2	71.5	56	116.6	0.46
W-pL13	CWP	6.6	37 °C	33.3	71	45	137.4	0.42
W-pL13	CWP	6.6	37 °C	40.5	116	64	117.4	0.33
ML308-pL13	WP	7.0	30 °C	11.7	68.5	51	42.7	0.13
ML308-pL13	CWP	6.6	37 °C	33.3	42.5	39	158.3	0.72
ML308-pL13	CWP	6.6	37 °C	35.9	74	62	106.3	0.44
W-pLOI297	CWPb	6.6	37 °C	37.8	71	63	110.7	0.52

^ain a 2.4-liter volume without filter-sterilization of the medium

^b 0.8 μm filter-sterilization of the medium