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. 2017 May 14;2017:2748192. doi: 10.1155/2017/2748192

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Copyright © 2017 Martina Kern et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Apoptosis (a) and cytotoxicity (b) of IPEC-J2 cells after treatment with bacterial strains (Ecf, ETEC, and Ecf + ETEC) or without bacteria (Con = control) as assessed by caspase-3/7 activity in luminescence assay for apoptosis and by a dead cell protease fluorescence assay for cytotoxicity [means ± SEM]. Measurements were taken after 6 h. Groups differ significantly (P ≤ 0.05) when marked with different letters, (e.g. a, b) in the figure. N = 4 (apoptosis assay) and N = 5 (cytotoxicity assay) independent experiments.