Figure 5.

Flow cytometry analysis and immunohistochemistry of liver-infiltrated myeloid cells in the WT and IL-33 KO following L2-MHV3-induced hepatitis. (a) Representative dot-plot of gated myeloid cells according to GR1, CD11b, and F4/80 staining. Macrophages are considered as GR1^interm/CD11b⁺/F4/80⁺ and neutrophils as GR1⁺/CD11b⁺/F4/80^low-stained cells. Presented dot plots are representative results observed in 5 different mice by conditions. (b) F4/80 immunostaining of paraffin-embedded mouse liver control (PBS) or infected with L2-MHV3 for 48 h or 72 h. (c) Quantification of F4/80-stained cells on 4 areas of 0.1 mm² by tissues and at least 3 different tissues by condition. (d) Ly6G immunostaining of paraffin-embedded mouse liver control (PBS) or infected with L2-MHV3 for 48 h or 72 h. (e) Quantification of Ly6G-stained cells (neutrophils) on 2 areas of 2 mm² by tissues and at least 3 different tissues by condition. Statistical analyses were done according to the Mann-Whitney U test. # represents significant difference with WT PBS condition, ^∗ represents significant difference between WT and IL-33 KO conditions, and $ represents significant difference between two conditions in the same background mice. ^#p < 0.05; ^##p < 0.01; and ^###p < 0.001.