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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: J Genet Genomics. 2016 Jun 4;43(9):555–563. doi: 10.1016/j.jgg.2016.05.007

Fig. 3. BZS1 positively regulates HY5.

Fig. 3

A: Quantitative RT-PCR analysis of HY5 and HYH expression levels in wild-type (Col), BZS1-SRDX and BZS1-YPF plants. Seedlings were grown under constant white light for five days. The actin2 gene was used as an internal reference. Significant differences from wild type are marked as asterisks, P < 0.01. B: Western blot analysis of HY5 protein accumulation in BZS1-SRDX and BZS1-YFP plants. The arrows indicate HY5 protein and the asterisks mark a non-specific band. Seedlings were grown under continuous white light for 4 days and total proteins were immunoblotted with anti-HY5 antibody. The loading controls were a non-specific band on the blot (left) and the Ponceau S staining of total proteins (right).