Fig. 4.

Influence of LPS injection on mRNA expression of ligands and receptors of the angiopoietin/Tie2 system and the VEGFA/VEGF-receptor system in mouse kidney and lung.

Mice were i.p. injected with LPS at 0.5 mg/kg (1,500 EU/g) bodyweight and sacrificed at 4, 8, and 24 h later. mRNA expression of Ang1 (A, B), Ang2 (C, D), Tie2 (E, F), VEGFA (G, H), VEGFR1 (I, J), and VEGFR2 (K, L) in kidney (A, C, E, G, I, K) and lungs (B, D, F, H, J, L) was determined using quantitative RT-PCR with GAPDH as housekeeping gene. Data are presented as mean ± SD (n = 5 animals/group). Significance P < 0.05.