Fig. 8.

Influence of incubation time on ligand binding competition experiments in the presence or absence of cediranib. HEK293T NLuc VEGFR2 cells were pretreated with either (a) vehicle or (b) 1 μM cediranib for 30 min at 37 °C prior to continued incubation in the presence of VEGF₁₆₅a-TMR (0.5–3 nM) and various concentrations of VEGF₁₆₅a for 10, 30 or 60 min at 37 °C. Data represent mean ± S.E.M from 3 to 4 independent experiments.