Fig. 10.

(a) Confocal imaging in HEK293T cells transiently expressing HaloTag VEGFR2. HEK293T cells stably expressing HaloTag VEGFR2 were treated with vehicle for 30 min at 37 °C. All cells were imaged live using a Zeiss LSM Exciter and are representative images from 3 independent experiments. (b). Structured Illumination Microscopy (SIM) super resolution images of HEK293T cells transiently transfected with HaloTag VEGFR2 (green) and treated for distinct time points with 3 nM VEGF₁₆₅a (red; 5, 30 or 120 min at 37 °C). Areas of colocalisation are shown in yellow and examples shown by the closed white arrow. Examples of non-localised intracellular HaloTag VEGFR2 are illustrated by the open white arrow.