Table 4.

Summary of NanoBRET derived binding affinity measurements when VEGF₁₆₅a-TMR and VEGF₁₆₅a are simultaneously co-incubated for set incubation times in the presence or absence of cediranib. Values are mean ± SEM of 3–4 separate experiments. pK_i values determined from individual experiments summarised in Fig. 8.

Ligand incubation time (min)	VEGF165a pKi
		Plus 1 μM cediranib
10	8.93 ± 0.09	9.17 ± 0.04
30	8.99 ± 0.10	9.23 ± 0.06
60	9.28 ± 0.06	9.18 ± 0.06