FIG. 1. miR-29s directly targeted MMP2 by interaction with the 3'-UTR of MMP2. (A) Predicted consequential pairing of the target region. The sequences of miR-29s binding sites within the 3'-UTR of MMP2 and their seed regions (bold). (B), (C) HT1080 cells were cotransfected with miR-29s mimics or control miRNA (50 nM) and the plasmid psiCHECK-2 (500 ng) containing the wild-type 3'-UTR of MMP2. After 48 h, luciferase assays were performed. Firefly luciferase was used to normalize transfections and eliminated the need to transfect a second vector control (^*p<0.05).