FIG. 3. miR-29s inhibited cell invasion in vitro. (A) Cellular invasion was evaluated in HT1080 cells using Matrigel Invasion Chambers with 8.0 mm PET membrane. Cells (1.0-104) transfected with miR-29s mimics were seeded with serum-free medium on transwell chambers precoated with Matrigel. Medium containing 1% fetal bovine serum was used as a chemoattractant. After incubation for 24 h, invaded cells were stained with toluidine blue. (B) The number of invaded cells was counted under a microscope. The data are presented as relative cell invasion (*p<0.05).
