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. 2017 May 4;5(6):961–967. doi: 10.1002/ccr3.986

Table 1.

Primer sequences used for MLC1 amplification and sequencing

Fragment Oligonucleotide primers Annealing temperature(°C) Length(bp)
Exon 2 5′‐CTCAGAGTGGCCAAAGCC‐3′ 60 328
5′‐ACCAGAGGGACCAGATGC‐3′
Exon 3 5′‐CAGAAGTTGAAGGGTCAGGG‐3′ 57 561
5′‐GAAGTTTCACTCTCATTGCCC‐3′
Exon 4/5 5′‐GCTCATGGGATTCCGGTT‐3′ 60 548
5′‐ TGTGGGTGTCAGGCGTCT‐3′
Exon 6 5′‐ GGTGGCGTGAGAAAGGCG‐3′ 60 222
5′‐CCCACCTCGCTCACCCTG‐3′
Exon 7 5′‐ AGTGCTGAGTCCCTGTGC‐3′ 60 363
5′‐GCAGTAACAAACTCCCCC‐3′
Exon 8 5′‐ GGTGGGTGTGTCCTATGG‐3′ 60 652
5′‐ GGTGACTCTCTGTCTGAA‐3′
Exon 9 5′‐ TACCCCTGCTTCCCTGCG‐3′ 60 396
5′‐ ACCCCACCTTCCTCATTG‐3′
Exon 10 5′‐ GAACCAGCTTGGGACTAT‐3′ 55 275
5′‐ GGGGGGCTCTGAAATAAA‐3′
Exon 11 5′‐ GCTCACACCTCCTTCCGC‐3′ 60 278
5′‐ CCCACCCCACAGGCTTCT‐3′
Exon 12 5′‐ GCAGGCGTTTCTGGGACA‐3′ 60 355
5′‐ GCTCAGGGCGATTAGGGG‐3′