Table 1.

Cellular and physiological characteristics of P. inhibens during growth with selected supply ratios of NH₄⁺ and PO₄³⁻ (for growth curves see Fig. S3, Supporting Information).

Physiological parameters	External N:P supply ratioa
	267 (P-limited)	67 (P-limited)	17 (NP-limited)	4 (N-limited)	1 (N-limited)
ODmax (600 nm)	3.24 ± 0.03	3.26 ± 0.04	0.92 ± 0.01	0.61 ± 0.02	0.58 ± 0.04
μexp [h−1]	0.163 ± 0.003	0.148 ± 0.003	0.225 ± 0.006	0.207 ± 0.003	0.250 ± 0.001
μlin [h−1]	0.090 ± 0.002	0.087 ± 0.002	0.040 ± 0.001	0.024 ± 0.001	0.025 ± 0.002
YGlc [g dry cells (mol Glc)−1]b	66.8 ± 1.2	63.3 ± 1.0	46.5 ± 1.2	63.0 ± 0.2	55.1 ± 0.2
[g dry cells (mol NH4+)−1]b	494 ± 18	493 ± 2	360 ± 12	315 ± 6	296 ± 2
[g dry cells (mol PO43−)−1]b	23 883c	21 185c	5393c	3178 ± 211	2,763 ± 46
Internal N:P ratio (biomass)d	36	43	16	12	13
Internal N:P ratio (biomass)b	46	57	19	11	12
Internal C:N ratio (biomass)d	18	19	14	11	10
Internal C:N ratio (biomass)b	30	26	22	16	17
Internal C:P ratio (biomass)d	661	821	233	129	129
Internal C:P ratio (biomass)b	1408	1460	420	183	197
TDA (Abs398nm) b	0.54 ± 0.01	0.58 ± 0.01	0.04 ± 0.00	0.03 ± 0.00	0.06 ± 0.00
TDA [nM] b	0.19 ± 0.05	0.24 ± 0.09	0	0	0

^aSelected molar N:P supply ratios with concentrations of NH₄⁺ and PO₄³⁻ (in brackets, respectively) in the medium: 267 (8.0 mM, 30 μM), 67 (2.0 mM, 30 μM), 17 (0.5 mM, 30 μM), 4 (0.5 mM, 125 μM) and 1 (0.5 mM, 0.5 mM).

^bValues at ∼OD_max (i.e. at the transition into stationary growth phase). Molar growth yields were calculated according to the following formula:

.

^cComplete consumption of PO₄³⁻ assumed (initial concentration below limit of quantitation [50 μM]; Ruppersberg et al.2016).

^dValues at ∼0.5 OD_max during linear growth.