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. 2017 May 24;8:15328. doi: 10.1038/ncomms15328

Figure 1. Loss of HCH1 relieves the Hsp82-W585T growth phenotype.

Figure 1

See also Supplementary Fig. 1. (a) In the image on the left, one protomer of yeast Hsp82 is in green, the other in grey (PDB 2CG9). Residues 582QFGWSANME590 are in blue, with W585 in red and A587 in orange. The missing loop (which is not resolved in this crystal structure) containing Y606—598LRDSSMSSYMSSK610—is shown as a dotted line in magenta. The image on the right is a magnification of the middle domain/C-terminal domain interface of the Hsp82 protomer shown in the box in the left panel. (b) hsc82hsp82 and hch1hsc82hsp82 cells expressing a WT copy of HSC82 were transformed with either WT or W585T HSP82 constructs. Transformants were struck on to 5-FOA for two days, grown overnight in selective media, diluted 10-fold and grown on selective media plates at the indicated temperatures for two days. (c) hsc82hsp82 cells expressing HSP82 WT or W585T were transformed with vector or pRS416GPD-HCH1. Resulting colonies were grown as described in b.