Figure 3. Hsp90-Y627 phosphorylation disrupts client interactions.

See also Supplementary Fig. 2. (a) 293 A cells were transfected with the pcDNA3-Flag vector or Flag-Hsp90α WT, Y627F or Y627E as indicated. Cells were collected, lysed and Flag complexes were isolated using anti-Flag resin and analysed using SDS–PAGE. (b) Transfections were performed and analysed as in a. Except a pLNCX-chick-v-SRC construct was co-transfected with each sample.