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. 2017 May 24;8:15328. doi: 10.1038/ncomms15328

Figure 4. The Hsp82-Y606E mutation complements Hch1 in vivo activity.

Figure 4

See also Supplementary Fig. 3. (a) hsc82hsp82 cells were transformed with Hsp82 WT, Y606F or Y606E constructs. The transformed cells were then grown on 5-FOA to lose the WT copy of Hsp90 in those strains and transformed with either vector or pRS416GPD-HCH1. The second set of transformants were then grown overnight in selective media, diluted 10-fold and grown for 2 days at the indicated temperatures. (b) hsc82hsp82 cells were transformed with the indicated HSP82 mutants and grown on 5-FOA for 2 days. Colonies grown on 5-FOA were then grown overnight in minimal media, diluted 10-fold and grown on YPD or 5-FOA plates for two days at 30°. (c) hsc82hsp82 cells transformed with the indicated mutants as well as vector or WT pRS416GPD-HCH1 were grown as in a on selective media.