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. 2017 May 24;8:15328. doi: 10.1038/ncomms15328

Figure 5. Hsp82-Y606E disrupts client protein expression and activity.

Figure 5

See also Supplementary Fig. 4. (a,b) hsc82hsp82 and hch1hsc82hsp82 cells expressing Hsp82 WT, Y606F or Y606E (MR923, MR925, CLY25, CLY29, CLY24 and CLY28 respectively) were transformed with plasmids containing either pG/N795-GR and pUCΔSS-26X (a) or pRS414GAL-Ste11ΔN and PRE-lacZ (b). Assays were performed in triplicate as indicated in the Fig. 2a,b. (c) HCH1 or hch1 cells expressing Hsp82 WT, Y606F or Y606E as indicated were transformed with pRS316GAL-v-SRC. Transformants were grown overnight in minimal media, diluted 10-fold and grown for 2 days at 30° on minimal media supplemented with glucose (URA D.O.) or galactose (URA-GAL). (d) Transformants from c. were grown overnight in Ura-RAF and switched to URA-GAL media overnight. Cells were lysed and analyzed as indicated.