Figure 2. Kinetics of Trm and effector CD8⁺T cells after i.n. immunization.

(a) Mice were i.n. immunized (prime (D0)–boost (D14)) with STxB-E7 and BALs and spleens were collected at day 21. Cells were stained and gated on living double-positive E7_39–47 tetramer and CD8⁺T cells to assess the expression of CD103 and CD49a. CD49a and CD103 double-negative cells were further stained with anti-CD62L mAb. Isotype controls were included in each experiment. (b) Absolute number of Trm cells (CD8⁺Tet⁺CD49a⁺CD103^–, CD8⁺Tet⁺CD49a^–CD103⁺, CD8⁺Tet⁺CD49a⁺CD103⁺) and effector CD8⁺T cells (CD8⁺Tet⁺CD49a^–CD103^-) in BALs measured at D0, D7, D30 and D90 after a vaccine boost. (c,d) Mice were i.n. immunized or not with STxB-E7 and 7 days after the boost grafted with the TC1 tumour cells. Three and six days after the graft, the percentage (c) and the absolute number (d) of Trm and effector CD8⁺T cells were measured in BALs. Non-immunized mice were also included as controls. Experiments were reproduced at least three times.