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Effector CD8+T cells from BAL and spleen and Trm from BAL were sorted 7 days after the boost i.n. immunization with STxB-E7. Cells were pulsed or not with a set of concentrations of the E739–47 peptide and 24 h later IFNγ Elispot was performed. Experiments were reproduced two times and statistical analysis was performed with a two-way analysis of variance (ANOVA). Error bars indicates s.e.m. NS, not significant.
