Figure 6. Trm controls orthotopic tumour growth as demonstrated in parabiosis experiments.

Mice (n=5) were i.n. immunized with STxB-E7 and 7 days after the boost submitted to parabiosis surgical procedure. (a) Dot plot of T cells stained with anti-CD8 and D^b-E7_39–47 tetramer in BALs or spleens from the immunized (left side panels) or non-immunized (right side panels) parabiont mouse 21 days after the surgery. Double-positive cells were gated for CD103 analysis from the BALs of the immunized mice (left). (b) Seven days after the parabiosis surgery, the immunized and non-immunized parabionts or naive mice (n=5 per group) were grafted in the tongue with TC1 cells and their survival was monitored. These experiments were independently reproduced three times with similar results. Statistical analysis used a Log-rank test. ***P<0.001.