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. 2017 May 24;8:15588. doi: 10.1038/ncomms15588

Figure 2. Transcriptome profiling coupled with EBE prediction reveals candidate target genes of Avrb6.

Figure 2

(a) Schematic diagram of experimental design to identify Avrb6 target genes in cotton. (b) Avrb6 protein expression in cotton protoplasts. Cotton protoplasts isolated from Ac44E were transfected with avrb6-HA or an empty vector as a control (Ctrl). Samples were collected 12 h after transfection and subjected to immunoblotting with α-HA antibody (top panel). Ponceau S. staining (Ponc.) of total protein served as the protein loading control; RuBisCo (RBC) is shown (bottom panel). (c) Avrb6 protein expression in cotton and N. benthamiana. Cotyledons from 2-week-old Ac44E cotton and leaves of 4-week-old N. benthamiana were infiltrated with Agrobacterium carrying 35S::avrb6-HA or an empty vector control (Ctrl). Inoculated tissues were collected at 48 hpi and subjected to immunoblotting. B and C were repeated three times with similar results. (d) Venn diagram of Avrb6-induced genes and genes with Avrb6 EBEs in G. raimondii genome. (e) The predicted EBE of Avrb6 RVDs. Coloured boxes on the top panel display the RVD of each repeat of Avrb6. Bottom panel indicates the relative frequencies of RVD associations with the four nucleotides for each repeat.