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. 2017 May 24;8:15526. doi: 10.1038/ncomms15526

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The graph shows the dependence of limonene production from glucose on the purge valve implemented at the Gap step. Various components were left out of each reaction (Gap, wild-type Gap from E. coli; mGap, NADP⁺-specific mutant Gap from G. stearothermphilus; NoxE, NADH oxidase from Lactococcus lactis). The last experiment (+Gap, +mGap and +NoxE) contains all components. Leaving out any component leads to reduced limonene production from glucose. All reactions were started with the addition of 250 mM glucose and analysed after 16 h. Reactions were performed in triplicate (n=3) and analysed as described in the Methods. Error bars represent s.d. ND, not determined.