Figure 5. High-titre production of multiple monoterpenes from glucose.

The chart shows production of monoterpenes limonene, pinene and sabinine from 500 mM glucose over 7 days. The final titre of monoterpenes and glucose remaining were determined by GC and enzyme assay as described in the Methods. For the limonene synthase reaction (green bar), the product was 100% limonene. For the pinene synthase reaction (purple bar), the product distribution is 80.8±0.33% α-pinene and 19.2±0.33% β-pinene. For the sabinene synthase reaction catalysed by the N345A Limonene synthase mutant (orange bar), the product distribution is 11.5±0.03% α-pinene, 11.2±0.01% β-pinene, 37.9±0.02% (−)-sabinene, 3.5±0.01% (+)-sabinene, 27.1±0.04% limonene and 5.7±0.01% myrcene. Reactions were performed in triplicate (n=3) and analysed as described in the Methods. Error bars represent s.d.