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Native protein gel electrophoresis of DhaK∷DhaR complexes. (A) Titration of DhaR(N) (5 μM constant concentration) with DhaK (0, 0.1, 1.0, 5.0 and 10 μM). (B) Dissociation of the 1:1 DhaK∷DhaR(N) complex (5 μM constant concentration) with increasing concentrations of DhaL∷ADP (0, 1.0, 2.5, 5.0, 10 and 20 μM). Lanes R, K, L, 5 μM DhaR(N), 10 μM DhaK, 20 μM DhaL.
