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Levels of nitrite and nitrate accumulated in a reaction buffer after 60 min incubation of XOR and the novel organic nitrate NDHP. The experimental conditions are as described in Fig. 2 and NADH (1 mM) was used as the electron donor. (n = 3–4 independent experiments for each NDHP concentration tested).
| NDHP | 0.1 mM | 1 mM | 10 mM |
|---|---|---|---|
| Nitrite (μM) | 7.13 ± (0.52) | 28.87 (4.65) | 53.70 (8.68) |
| Nitrate (μM) | 9.33 (0.75) | 26.42 (0.84) | 104.7 (9.96) |
Data are shown as mean ± (S.E.M.)
