Figure 1.

CSWKYWFGEC Peptide Probe Identifies ASC in Mouse WAT and Tumors

Prostate adenocarcinoma RM1 allografts grown in immunocompetent mice treated with PBS (A and C) or ASC-targeting peptide D-WAT (B and D). WAT (A and B) and tumor (C and D) sections were incubated with biotinylated ASC-binding peptide CSWKYWFGEC and then subjected to immunofluorescence with antibodies specific for mouse PDGFRβ (red) to localize ASCs and with streptavidin (green) to localize the CSWKYWFGEC peptide. Note CSWKYWFGEC signal on PDGFRβ-expressing ASCs in control WAT and tumors is shown (arrows). Nuclei are blue. The scale bar represents 50 μm. (E) Quantification of data from (A)–(D) shows that the frequency of CSWKYWFGEC-bound cells in WAT and tumor capsule is reduced upon D-WAT peptide treatment. Plotted are mean ± SEM for multiple fields (n = 10). *p < 0.01 (Student’s t test). Experiments were performed twice with similar results.