VirE3 interacts with VirE2 and AtKAPα in Y2H assay. (A) Cell growth in the absence of histidine. (B) β-galactosidase assay. (C) Cell growth in the presence of histidine. Lane 1, VirE3+AtKAPα; lane 2, VirE3+VirE2; lane 3, VirE3+VirD2; lane 4, VirE3+lamin C. VirE3-mNLS12 interacts with VirE2, but not with AtKAPα in the Y2H assay. (D) Cell growth in the absence of histidine. (E) β-Galactosidase assay. (F) Cell growth in the presence of histidine. Lane 1, VirE3-mNLS12+AtKAPα; lane 2, VirE3-mNLS12+VirE2.