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VirE3 facilitates nuclear import of VirE2 in COS-1 cells: (A) GFP-VirE2; (B) GFP-VirE3; (C) GFP-VirE2+VirE3; (D) GFP-VIP1; (E) GFP-VirE2+VIP1; (F) GFP-VirE2+VirE3-mNLS12; (G, H) YFP-VirE2+CFP-VIP1; (I, J) YFP-VirE2+CFP-VirE3; and (K, L) YFP-VirE2+CFP-VirE3-mNLS12. YFP is in green (G, I, and K), and CFP is in blue (H, J, and L). Asterisks indicate cell nuclei that contain VirE2. All images are single confocal sections.
