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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Nat Chem. 2017 Mar 6;9(4):325–332. doi: 10.1038/nchem.2739

Figure 2. RPR activation by homopolymeric peptides.

Figure 2

Top: the amino acids Lysine (K), Ornithine (Orn) and Diaminobutyric acid (Dab) (top panels) have progressively shorter amino group side chains. Below: PAGE of primer extensions on template TI by the Z RPR over 3 days at 17°C, alone or with indicated peptides of different (a) lengths, (b) concentrations and (c) side chains and chirality. d, EMSA: The fluorescently labeled Z ribozyme (Z-Fluor 647) was incubated with K10 peptide at the indicated concentrations with 22 mM [Mg2+], and resolved on a non-denaturing agarose gel.