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. 2017 Feb 16;8(19):30766–30780. doi: 10.18632/oncotarget.15403

Figure 1. DNA vaccination induces the functional memory CD8+T cells.

Figure 1

BALB/c mice were injected with NY-ESO-181-88 peptide emulsified in IFA with or without CpG ODN or plasmids encoding the entire NY-ESO-1 protein, using a gene gun. A., B. At 44 days after the last immunization, CT26-NY-ESO-1 was inoculated into the immunized mice. At 14 days after tumor inoculation, splenocytes from the mice were incubated with NY-ESO-181-88 or control peptide, and cytokine secretion was analyzed by flow cytometry. Production of TNF-α and IFN-γ was negligible, when the splenocytes were stimulated with the control peptide. (Blue filled; DNA vaccine, Red filled; peptide vaccine, black line; isotype control). C. Tumor sizes were monitored twice or thrice per week (n = 5/group). D. At 44 days after the last immunization, the expression of CD44, CD62L and IL-7R on vaccine-specific CTLs cell was analyzed by flow cytometer. E. At 44 days after the last immunization, splenocytes were labeled with CFSE and stimulated with mERK2 peptide for 3 days and proliferation activity was analyzed by flow cytometer.