Skip to main content
. 2017 Feb 16;8(19):30766–30780. doi: 10.18632/oncotarget.15403

Figure 2. STAP2 regulates vaccine-induced memory CD8+ T cell differentiation.

Figure 2

A. At 7 days after the last vaccination, vaccine-specific CTLs were collected from draining the LNs, and RT-PCR was performed to quantify STAP2 mRNA levels. B. Splenocytes derived from DUC18 mice were stimulated with mERK2 peptide for 3 days and then cultured in rIL-2 (10 or 50 IU/ml)-containing medium for 4 days. STAP2 expression in cultured cells was measured by RT-PCR. C. CD8+ T cells derived from DUC 18 mice were infused in BALB/c mice. Mice were then vaccinated with mERK2-coding plasmid DNA and infused cells were collected on the indicated days. STAP2 expression was measured by RT-PCR. D. On d-7 after antigen stimulation, IL-7R positive and negative cells were sorted, and analyzed by RT-PCR for STAP2 expression. E. On day 7 after the last vaccination, IL-7R positive and negative cells were sorted from the infused mice and analyzed by RT-PCR for STAP2 expression. The results are representative of two to four experiments. Data are expressed as the mean ± SD. *p < 0.05 is considered significant.