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. 2017 Feb 16;8(19):30766–30780. doi: 10.18632/oncotarget.15403

Figure 3. STAP2 has a crucial role in memory generation but not in effector cell differentiation in the spleen.

Figure 3

Splenocytes derived from DUC18 mice were stimulated with mERK2 peptide for 3 days, transduced with STAP2-specific siRNA, scrambled control siRNA, or GFP mRNA by electroporation, and injected into BALB/c mice. A. Transfection efficacy of GFP mRNA and knockdown efficacies of STAP2 mRNA were examined by flow cytometric analysis and qRT-PCR, respectively. At 4 or 44 days after infusion, B., C., D. the number of IFN-γ+ T cells, the expression of memory markers (KLRG and IL-7R), and IFN-γ and TNF-α production were examined. (Gray filled; scrambled siRNA, Red line; STAP2 siRNA, black line; non-immunized control). The results are representative of two to four experiments. Data are expressed as the mean ± SD. *p < 0.05 is considered significant.