Skip to main content
. 2017 Feb 16;8(19):30766–30780. doi: 10.18632/oncotarget.15403

Figure 4. Importance of STAP2 on the elicitation of functional CD8+ memory T cells by in vivo priming.

Figure 4

Naïve CD8+ cells derived from DUC18 mice were transduced with STAP2-specific siRNA, scrambled control siRNA, or with GFP mRNA by electroporation, and injected into BALB/c mice. These BALB/c mice were then immunized with plasmids encoding mERK2 using a gene gun. A. At 2 days after transfection, expression of GFP and the knockdown efficacy of STAP2 mRNA was examined by flow cytometric analysis and qRT-PCR, respectively. B., C., D., E. At 44 days after vaccination, the number of IFN-γ+ T cells, the expression of memory markers (KLRG and IL-7R), the production of IFN-γ and TNF-α and proliferation were examined. (Gray filled; scrambled siRNA, Red line; STAP2 siRNA, black line; non-immunized control). The results are representative of two to four experiments. Data are expressed as the mean ± SD. *p < 0.05 is considered significant.