Figure 6. STAP2-mediated augmentation of secondary memory CD8⁺ T cell responses.

A. Splenocytes derived from DUC18 mice were stimulated with mERK2 peptide for 3 days, transduced with STAP2-specific or scrambled control siRNA by electroporation, and infused into BALB/c mice. At 44 days after infusion, CMS5a cells were subcutaneously inoculated into these mice and then collected CD8⁺ T cells were subjected to cytokine production assay. B. BALB/c mice were infused with siRNA-transduced DUC18 CD8⁺ cells and then immunized with plasmids encoding the mERK2 by gene gun. At 44 days after the final immunization, CMS5a were subcutaneously injected into these mice and splenic IFN-γ and TNF-α production was measured. C. CMS5a growth was monitored in (A) (n = 5/group). The results are representative of two to four experiments. Data are expressed as the mean ± SD. *p < 0.05 is considered significant.