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. 2017 Feb 16;8(19):30766–30780. doi: 10.18632/oncotarget.15403

Figure 7. Attenuation of STAT3/SOCS3 cascade in antigen-stimulated CD8+ T cells by STAP2 knockdown.

Figure 7

BALB/c mice were infused with DUC18 CD8+ T cells and then immunized with plasmids encoding mERK2 by gene gun. A. At the indicated days, pSTAT3, pSTAT5, SOCS3, and Eomes expression in the splenocytes of DUC18 CD8+ T or in endogenous CD8+ T cells was measured by flow cytometry. (B, C) BALB/c mice were infused with siRNA-transduced DUC18 CD8+ cells and then immunized with plasmids encoding mERK2 by the gene gun. B. At 4 days after immunization, SOCS3 and Eomes expression in the infused cells was measured by flow cytometry. C. SOCS3 and Eomes expression in infused cells was measured after IL-10 and IL-21 stimulation. D. BALB/c mice were infused with the indicated siRNA- and/or mRNA-transduced DUC18 CD8+ T cells and then immunized with plasmids encoding mERK2 by the gene gun. At 44 days after injection, CMS5a were inoculated subcutaneously into these mice and IFN-γ and TNF-α production was examined. The results are representative of two to four experiments. Data are expressed as the mean ± SD. *p < 0.05 is considered significant. n.s. = not significant.