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. 2005 Jan 6;24(2):270–282. doi: 10.1038/sj.emboj.7600530

Figure 1.

Figure 1

Size exclusion chromatography of T. thermophilus wild-type Soj and Soj D44A in the presence and absence of ATP. The elution volume of each species is indicated in millilitres above the respective trace. (A) In the absence of ATP, wild-type Soj elutes as a monomeric species (black). In the presence of ATP, Soj elutes as a dimer–monomer equilibrium (green). (B) Soj D44A, deficient in nucleotide hydrolysis, also elutes as a monomer in the absence of ATP (black), but elutes almost solely as a dimer in the presence of ATP (green). Red: absorbance at 260 nm indicating the elution volume of ATP and the presence of ATP in the dimeric protein.